Gulmez, M.Arsoy, D.Betmezoglu, M.2024-12-242024-12-2420202313-626X2313-3724https://doi.org/10.21833/ijaas.2020.10.013https://hdl.handle.net/20.500.12604/7609Wheat straw predigestion is needed for the industrial bioprocess and ruminant feeding. We aimed to search for some predigestion conditions for wheat straw. 500ml of water was used as a moistener for each 100g of the chopped straw sample. For each sample, 2g NaOH as alkalizer; 3g citric acid as neutralizer; a mix of 1g KH2PO4, 1g (NH4)SO4, 0.3g CaCl2, 0.2g MgSO4, 2g powdered molasses and 1g inactive dry yeast as supplements were used. Inoculation culture was non-selected total flora of rumen remained live after consecutive treatments of 30 min of alkali (NaOH, pH 10) and 30 min of acid (3% citric acid, pH 4) environments. The samples were incubated aerobically at 37 degrees C for 14 days, and the analyses were made at days 0, 7, and 14. Hot alkali and neutralizer determined to be the most effective on predigestion when used consecutively. The 15 to 20% increases were determined from the water-soluble mass of the samples incubated for 7 days. No net effect of the supplements and inoculant on the predigestion was determined. From the dominant aerobic microflora of the predigested samples, some opportunistic and/or sporadic pathogenic aerobic Gram+ ve bacteria were identified by using VITEK 2 GP identification system. As a result, it was determined that 2g NaOH in hot water for 7 days of microbial predigestion of 100g chopped straw appeared to be sufficient. Nevertheless, the hygiene aspect of such bacteria in the predigestion applications have to be intensively investigated in further studies. Acceptability, digestibility, and rumen health effects of such predigested straw have also to be tested when it was aimed to prepare feed materials. (C) 2020 The Authors. Published by IASE.eninfo:eu-repo/semantics/openAccessWheat strawMicrofloraPredigestionWater-solublesArticle710117124N/AWOS:000814035900012Q32-s2.0-8515100800010.21833/ijaas.2020.10.013