Karagecili, HasanDemirdag, RamazanYerlikaya, Emrah2024-12-242024-12-2420211018-46191610-2304https://hdl.handle.net/20.500.12604/8033Carbonic anhydrases (CAs) take major roles in many pathological situations associated with convulsions, carcinoma, glaucoma, weight problems, etc. In former researches; using tissues and erythrocytes of a wide range of organisms like; dogs, swine, sheep, chickens, bees, fish, goats, bovines, bacteria, and humans, CA enzymes have been purified and characterized. With this research, the CA enzyme has purified from Alectoris chukar liver tissue with 1.23 EU x mg(-1) of specific activity, 55.5% purification yield, and 460.7 purification folds. The purity of the purified enzyme has been approved by SDS-PAGE. The molecular weight was estimated to be about 28.4 kDa. As the characterization of CA enzyme's in the Alectoris chukar liver has been done; the optimum ionic strength-50 mM Tris-SO4. the optimum pH-8.0, and the optimum temperature-35 degrees C have been decided. K-M and V-max values were calculated by Lineweaver Burk graph. K-M constants were estimated as 1.035 mM, V-max, values as 0.02 molxmin(-1) for 4-nitrophenyl acetate. Inhibitory consequences of some pesticides (Spinosad and Dimethoate) and metal ions (Al3+, Cu2+, Ba2+, Fe2+, Mn2+, Se2+) have been analyzed on the purified CA enzyme. Inhibition graphics were drawn to find the IC50 values of metals and pesticides displaying inhibition.eninfo:eu-repo/semantics/closedAccessPartridgeInhibitionPollutionEnvironmentCatalysisArticle3011A1262012628Q4WOS:000716443600062